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New PDF release: Amino Acid Analysis Protocols (Methods in Molecular Biology

By Cooper C., Packer N.

ISBN-10: 0896036561

ISBN-13: 9780896036567

ISBN-10: 1592590470

ISBN-13: 9781592590476

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Additional info for Amino Acid Analysis Protocols (Methods in Molecular Biology Vol 159)

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299– 306. 10. Cohen, S. A. and De Antonis, K. M. (1994) Applications of amino acid analysis derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate: Analysis of feed grains, intravenous solutions and glycoproteins. J. Chromatog. 661, 25–34. 11. Van Wandelen, C. and Cohen, S. A. (1997) Using quaternary high-performance liquid chromatography eluent systems for separating 6-aminoquinolyl-N- Precolumn Derivatization with AQC 47 hydroxysuccinimidyl carbamate-derivatized amino acid mixtures.

Grams for the standard mixture containing carboxymethylcysteine (CMCys) and for an S-carboxymethylated recombinant antibody sample are given in Fig. 7A,B, respectively. 3. Monitor CMCys and half-cystine residue/mol values to determine the extent of cysteine alkylation (see Notes 26 and 27). 0 Sample injection Buffer change Buffer change Reagent pump Buffer change Reagent pump Recyle (start next run) Li-A buffer, 38°C Li-A to Li-D Li-D to Li-R Ninhydrin to water Li-R to Li-A Water to ninhydrin Buffer pump: 20 mL/h.

West, K. A. and Crabb, J. W. (1992) Application of automatic PTC amino acid analysis, in Techniques in Protein Chemistry III (Angeletti, R. ), Academic, London and New York, pp. 233–242. Purification of Proteins for AAA 37 4. , Mische, S. M. (1992) Amino acid analyis of PVDF bound proteins, in Techniques in Protein Chemistry III (Angeletti, R. ), Academic, London and New York, pp. 249–260. 5. Gilman, L. B. and Woodward, C. (1990) Hydrolysis methods in current research, in Protein Chemistry (Villafranca, J.

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Amino Acid Analysis Protocols (Methods in Molecular Biology Vol 159) by Cooper C., Packer N.

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