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Download e-book for kindle: Advances in Genetics, Vol. 1 by M. Demerec (Ed.)

By M. Demerec (Ed.)

ISBN-10: 0120176017

ISBN-13: 9780120176014

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2. 3. 4. 5. 0. N,N,N′,N′-tetraethylenediamine (TEMED). 10% Ammoniumpersulphate. 25% xylene cyanol, 30% (v/v) glycerol. 6. Kodak X-Omat film. 5. Elution of DNA 1. 2. 3. 4. 2. Glycogen (10 mg/mL) (Sigma). 100% Ethanol. 85% Ethanol. 6. Southern Blotting 1. 2. 3. 4. 5. 6. 7. 8. 9. Agarose (electrophoresis grade) (Gibco BRL). 10X TBE. Ethidium bromide (10 mg/mL). 125 M HCl. 5 M NaOH. 5 M Tris-HCl. Hybond N nitrocellulose membrane (Amersham Pharmacia). α-32P dCTP: (specific activity 3000 mmole/µCi). 1 M polyvinyl pyrrolidone, 10 mg/mL bovine serum albumin (BSA) portion V (Sigma).

153–168. Tommasi, S. and Pfeifer, G. P. (1995) In vivo structure of the human cdc2 promoter: release of a p130/E2F-4 complex from sequences immediately upstream of the transcription initiation site coincides with induction of cdc2 expression. Mol. Cell. Biol. 15, 6901–6913. Tornaletti, S. and Pfeifer, G. P. (1995) UV-light as a footprinting agent: modulation of UV-induced DNA damage by transcription factors bound at the promoters of three human genes. J. Mol. Biol. 249, 714–728. Chemical DNA Sequencing 41 18.

4. Load only one half of the sample or less using a very thin flat tip. 5. Run the gel until the xylene cyanol marker reaches the bottom. Fragments below the xylene cyanol dye do not hybridize significantly. 6. , the bottom 40 cm of it) to Whatman 3MM paper and cover with Saran Wrap. 7. Electroblotting of the gel piece is performed with a transfer box available from Owl Scientific (see Note 7). Pile three layers of Whatman 17 paper, 43 × 19 cm, presoaked in 90 mM TBE, onto the lower electrode. Squeeze the paper with a 38 8.

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Advances in Genetics, Vol. 1 by M. Demerec (Ed.)


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